Second-base umpire Chris Conroy called out Escobar, who slid head-first into the base. Escobar bounced up and gave the safe signal to the dugout. That prompted Maddon to walk onto the field and issue his challenge.
After a two-minute, 17-second review, the umpires ruled that the call stands.
"Again, I think that was decided based on non-conclusive evidence," Maddon said. "Based on my perspective, and even from Yuni, I thought he had tagged him high on the back on the armpit and his arm was in."
The Rays have had 20 challenges this season. Six have been overturned, six have had the call stand, and eight have been confirmed.
Bill Chastain is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.