The 30-year-old ran 10 bursts of about 120 feet before Saturday's matchup with the Blue Jays and said he felt fine.
"I didn't want to push it too hard, but everything went smoothly," Fuld said. "I'd say I went about 55 to 60 percent."
The injury originally occurred Sept. 16 when Fuld tried to beat out a grounder in the fifth inning of a 6-4 loss to the Yankees. As he crossed first base, he grabbed the back of his leg. An MRI later revealed a moderate strain.
Prior to his current injury, Fuld said he had never had a hamstring injury.
Fuld took batting practice in the cages Saturday and said he would continue to do so. Moving forward, he plans to run again Sunday, take Monday off and resume his testing Tuesday, practicing his running out of the batter's box.
He missed extended time earlier in the year because of left wrist surgery and has played in only 39 games for the Rays this season, batting .273 with seven steals.
Fuld is confident he will be back before the end of the season.