Scott, who last appeared in a game on July 20, was hitting .225 with 12 homers and 45 RBIs on the season. He went 1-for-4, including a leadoff double in his first at-bat, in the Rays' 1-0 loss to the Royals on Tuesday.
"It feels good, it's been a long time," Scott said before the game. "I've been champing at the bit, I've run out of fingernails to chew on. I'm excited to get back and try and do my part to help the team win."
Scott went on a rehab assignment with Class A Advanced Charlotte on Aug. 11 and appeared in eight games, playing some time in the field at first base. He hit .308 with two home runs and six RBIs for the Stone Crabs.
Meanwhile, the Rays had been rolling, going 19-9 in his absence, including a five-game winning streak entering Tuesday's action.
"It's pleasing to see the team come together on all cylinders," Scott said. "It's very encouraging. We've always known that we've been capable of doing this, and to see that it's starting to happen, it's encouraging."
Evan Longoria had been seeing time as the designated hitter while recovering from a hamstring injury, but played third base on Tuesday.
Scott said he would feel fine playing first base and would be willing to play left field but due to a prior shoulder surgery, would probably be able to only able to throw it to a cutoff man.
The Rays have had a bevy of injuries this year. Pitcher Jeff Niemann, who is on a rehab assignment, is likely to be the next player off the disabled list. Brandon Guyer and Robinson Chirinos are also on the DL, but are expected to miss the rest of the season.
To make room on the active roster for Scott, Tampa Bay optioned infielder Sean Rodriguez to Triple-A Durham, but he will likely be called up Sept. 1 when rosters expand.
Rodriguez was hitting .215 and led the team with 14 errors.
"It's just one of those really difficult decisions to make," Rays manager Joe Maddon said of Rodriguez. "Sean is a pro, he understood, and he's gonna definitely use it to his benefit regarding to the adjustments he's made. Now he gets a chance to play every day."
Greg Zeck is an associate reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.