The Rays officially announced the sellout at 4 p.m. ET on Monday, which is the earliest sellout in team history, save for the inaugural home opener in 1998. Tropicana Field's capacity is now 36,048.
According to team spokesman Chris Costello, the only seats still available are a limited number of obstructed-view seats, scattered singles, and handicapped access seating.
The sellout is the third consecutive time the Rays have sold out the home opener since principal owner Stuart Sternberg's ownership group took control prior to the 2006 season.
"It is an encouraging indication of the community's excitement for the Rays, and it's a great way to begin the 2008 home season," said Rays president Matt Silverman.
Tickets are available for the remaining dates of the eight-game homestand at raysbaseball.com, the Tropicana Field Box Office and the Rays Tampa Office & Pro Shop.
Bill Chastain is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.